Page 81 - 2017地大报告电子书

环境学院发展报告

12.Functional Enzyme-Based Approach for Linking Microbial Community Functions
with Biogeochemical Process Kinetics
作 者 : Li Minjing; Qian Wei-jun; Gao Yuqian; Shi, Liang;Liu Chongxuan*

ENVIRONMENTAL SCIENCE & TECHNOLOGY 卷 : 51 期 : 20 页 : 11848-
11857 出版年 : OCT 17 2017

摘 要 : The kinetics of biogeochemical processes in natural and engineered environmental
systems is typically described using Monod-type or modified Monod-type models. These

models rely on biomass as surrogates for functional enzymes in microbial communities
that catalyze biogeochemical reactions. A major challenge of applying such models is

the difficulty of quantitatively measuring functional biomass for the constraining and
validation of the models. However, omics-based approaches have been increasingly used to

characterize microbial community structure, functions, and metabolites. Here, we propose
an enzyme-based model that can incorporate omics data to link microbial community functi

ons with biogeochemicalprocess kinetics. The model treats enzymes as time-variable catalysts
for biogeochemical reactions and applies a biogeochemical reaction network to incorporate

intermediate metabolites. The sequences
of genes and proteins from metagenomes,

as well as those from the UniProt
database, were used for targeted

enzyme quantification and to provide
insights into the dynamic linkage

among functional genes, enzymes, and
metabolites that are required in the

model. The application of the model
was demonstrated using denitrification, as an example, by comparing model simulations with

measured functional enzymes, genes, denitrification substrates, and intermediates.
论文链接：https://doi.org/10.1021/acs.est.7b03158

13.Hydrological connectivity from glaciers to rivers in the Qinghai-Tibet Plateau:

roles of suprapermafrost and subpermafrost groundwater
作 者 : Ma Rui*; Sun Ziyong*; Hu Yalu; Chang Qixin ; Wang Shuo ; Xing Wenle ;Ge
Mengyan

HYDROLOGY AND EARTH SYSTEM SCIENCES 卷 : 21 期 : 9 页 : 4803-4823 出版

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